The successful cardiac transplantation depends partly on the donor heart preservation by a solution that will ensure recovery of myocardial function. The purpose of this study was to perform the evaluation of various preservation solutions and to
accumulate the data on the requisites for ideal preservation solution.
The experimental setup was the constant pressure Langendorffs perfusion system. Isolated rabbit hearts were perfused for 20minutes with warm Krebs-Henseleit solution, stored for 4 hours in cold preservation solution after cardioplegia, and then
were
reperfused for 20minutes.
The 4 experimental groups were prepared : Hartmann's solution group(group 1, control), modified Euro-collins solution group(group II, MEC), modified University of Wisconsin group(group III, MUW), and CK solution(made by the author) group(group
IV,
CK).
The parameters for assessing the preservation ability were levels of enzymes in freezed myocardial tissues(lactate, creatine kinase-MB and adenosine deaminase), coronary flow, left ventricular developing pressure and dp/dt.
In conclusion, the ability of preservation for isolated rabbit heart was excellent in CK solution and modified University of Wisconsin solution, and poor in modified Euro-collins solution, compared with Hartmann solution. CK solution has low
potassium
concentrations(34.2mEq/L) and includes various substrates to be salutary on myocardial preservation. This fact may indicates the necessity of further refinements in selection or composition of electrolytes and substrates.
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